## Instruction for Enzyme Control Flux (ECF)

ECF is an algorithm for the estimation of a flux distribution of genetic mutants by integrating enzyme activity into Elementary Modes (EMs).

**Reference**

Hiroyuki Kurata, Quanyu Zhao, Ryuichi Okuda, Kazuyuki Shimizu: Integration of enzyme activities into metabolic flux distributions by elementary mode analysis, BMC Syst Biol, 1:31, 2007.

**Brief instruction**

**Program**: ecfexample.mat (Matlab file)

In Matlab

>> ecfexample

This program is an example for pyk mutant of E. coli.

Input data:

EM matrix, enzyme activity ratio, Elementary Mode Coefficients (EMCs).

Output data:

fluxcalu, fluxmean.

EmMatrix is the elementary mode matrix; reactions*EMs;

ramdaMatrix is the elementary mode coefficients vector; EMs*(2*EMs);

ns is the No. of substrate uptake reaction in stoichiometric matrix;

sflux is the flux of substrate uptake;

enzyme is the relative enzyme activity ratio of mutant versus wild type;